Ghd7-cfp

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August undefined, 2024

WebSep 14, 2024 · The Ghd7 was used as a cell nucleus localization marker [ 40, 41 ]. The results showed that the YFP signals of both 35S::D5RF.1–YFP and 35S::D5RF.2–YFP were mainly observed in the nucleus ( Figure 3 a,b). Figure 3. Subcellular localization assay for D5RF.1 and D5RF.2 proteins. WebOur analysis revealed a regulatory network that may provide an approximation to the genetic constitution of the optimal heterosis observed in this hybrid. In this network, Ghd7 works as the core element, and three other genes, Ghd7.1, Hd1, …

OsELF3 Is Involved in Circadian Clock Regulation for Promoting ...

WebEnhanced expression of Ghd7 under long-day conditions delays heading and increases plant height and panicle size. Natural mutants with reduced function enable rice to be … forensic science asu online

A stress-responsive NAC transcription factor SNAC3 confers heat …

WebThe nuclei were identified by co-expressing the nuclear marker Ghd7-CFP with both 35S:SPL13-GFP and 35S:GFP. Fluorescence images were acquired using a confocal laser scanning microscope (Leica TCS SP2, MRC Centre for Regenerative Medicine, The University of Edinburgh, Edinburgh, UK) after incubating the protoplasts at 28 °C for … WebJan 1, 2024 · The vector 35S:GHD7:CFP was used as reference as its located in the nuclear. These two vectors were co-transformed into Arabidopsis protoplast for NnTT8 subcellular localization analysis according to the method described by Xiong et al. (2024). WebMar 1, 2011 · To assay the sub-cellular localization of GHD8, the coding sequence of GHD8 was fused to yellow fluorescent protein (YFP); in contrast, GHD7, which is a nuclear protein, was fused to cyan fluorescent protein (CFP). Both fluorescent proteins were individually driven by the constitutive 35S cauliflower mosaic virus promoter. forensic science assam

OsELF3 Is Involved in Circadian Clock Regulation for Promoting ...

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WebADH7. A gene on chromosome 4q23-q24 that encodes class-IV alcohol dehydrogenase 7 mu or sigma subunit, which like other alcohol dehydrogenases metabolises various … WebLooking for HARTELL, Low Profile/Plenum Rated, Condensate Removal Pump? Find it at Grainger.com®. With over 1.6M products and 24/7 customer service we have supplies … forensic science articlesWebSep 16, 2016 · Ghd7, which is a very important CO-like gene controlling grain number, plant height, and heading date in rice ( Xue et al., 2008 ), also regulates stress tolerance. The … forensic science autopsy webquest

"WebOct 30, 2024 · When an unrelated nuclear localization marker tagged by CFP (Ghd7-CFP) was used, 71 ± 3% or 84 ± 8% of transformed cells determined by the marker showed … " - Ghd7-cfp

Ghd7-cfp

Alternative functions of Hd1 in repressing or promoting heading …

WebA citrus pulp mastication trait-related ABSCISIC ACID-INSENSITIVE 5-like protein promotes fruit softening via transcriptionally activating pectate lyase gene - ScienceDirect Scientia Horticulturae Volume 314, 15 April 2024, 111933 WebSep 20, 2024 · Ghd7 is a well-known protein that regulates heading date and yield in rice, and localizes in the nuclear compartment (Xue et al. 2008 ). Ghd7- cyan fluorescent …

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WebSep 1, 2024 · To determine the subcellular localization of AtWRKY47, we co-expressed 35 S:WRKY47-GFP (green fluorescent protein) with the nuclear marker 35 S:Ghd7-CFP (cyan fluorescent protein) in Arabidopsis protoplasts. Nuclear localization of AtWRKY47 was observed since GFP fluorescence completely overlapped with CFP signal (Fig. 2 e). WebMar 1, 2011 · Ghd7, which encodes a CCT-domain-containing transcription factor, was reported to regulate rice grain yield, plant height, and heading date. The natural variation …

WebMar 14, 2024 · GHD7, a nuclear protein, was fused to cyan fluorescent protein (CFP) and co-expressed with OsMFT2-GFP in rice protoplasts. OsMFT2-GFP was co-localized with the GHD7-CFP in the nucleus (Figure 2b ), indicating that OsMFT2 is a nuclear protein. Knock-out and overexpressing OsMFT2 altered ABA sensitivity in seed germination and post … WebJan 1, 2013 · It was confirmed that Ghd7 is negatively regulated by Hd17 and Ef7, the same genes with OsELF3, by recent publications ( Matsubara et al., 2012; Saito et al., 2012 ). However, how the Hd17 / Ef7 affects circadian clock genes and further disrupts the photoperiod pathway was not well elucidated.

WebReceive General Hardwood Company Liquidation Offers. 7201 East McNichols Rd. Detroit, MI 48212. Toll Free 800.239.6077. Local 313.365.7733. Fax 313.365.9696 WebJan 7, 2015 · Ghd7.1 encodes a PRR37-like protein that represses flowering by suppressing the expression of the downstream genes Ehd1 and Hd3a under LD, greatly enhancing grain productivity 23. All these...

WebMay 28, 2014 · Three transient expression vectors were constructed: 35S::VirD5-YFP, 35S::VirD5C-YFP and 35S::VirD5N-YFP. The empty yellow fluorescent protein (YFP, as control) and the construct YFP vector plasmid DNA were used to cotransform rice protoplasts with 35S::Ghd7-CFP. The 35S::Ghd7-CFP was used as a nuclear …

WebNational Center for Biotechnology Information did wolf of wall street winWebAug 25, 2015 · Representative cells are shown, as imaged by confocal laser scanning microscopy. a, e, and i, Ghd7:CFP localization in the nucleus (blue fluorescence); b, detection in rice protoplasts of YN:WOX11 and ERF3:YC interaction, shown as yellow signal; f and j, as negative controls with YN:pVYNE (R)/pVYCE (R):YC and YN: pVYNE … forensic science articles 2020WebThe 35S::CFP-Ghd7 was used as the nucleic marker. The confocal image was observed using a confocal laser scanning microscope. 2.3. VirD5 Can Activate the Promotor of D5RF.1 To test whether VirD5 can bind to the promoter of Arabidopsis D5RF.1, we performed one-hybrid assays in yeast and dual-luciferase assays in Nicotiana benthamiana. forensic science atu sligoWebOct 1, 2024 · During Agrobacterium (Agrobacterium tumefaciens) infection, the translocated virulence proteins (VirD2, VirE2, VirE3, VirF and VirD5) play crucial roles. It is thought that, through protein–protein... forensic science atar scoreWebJan 31, 2024 · GHD7 and GHD8 are identified with a pleiotropic effect on grain number, plant height and heading date in rice. Both genes delayed flowering through inhibiting the … forensic science average salaryWeb发现spl13-gfp蛋白完全位于细胞核上，这是基于spl13-gfp和ghd7-cfp发射的荧光的完全重叠。相反，从游离GFP发出的荧光分布在整个细胞中。基于这些观察结果，我们得出结 … forensic science assignment helpWebGhd7 was used as a nuclear localization marker. from publication: GmFLD, a soybean homolog of the autonomous pathway gene FLOWERING LOCUS D, promotes flowering … forensic science average salary in uk